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The Streptamer technology is a novel method to isolate and characterize functional antigen-specific T-cells. Streptamers enable for the first time a reversible, antigen-specific staining of T-cells, leaving the isolated T-cells phenotypically and functionally indistinguishable from untreated cells.
The Streptamer approach is based on a reversible staining that utilizes IBA's core Strep-tag technology. MHC/peptide complexes fused with a Strep-tag sequence are generated and multimerized in the presence of Strep-Tactin. This Streptamer complex is ideally suited to bind and isolate cognate T-cells by fluorescent (FACS) or magnetic cell sorting. A key aspect of the Streptamer approach is the ability to rapidly remove the staining reagent after the isolation step by disassembly of the MHC/Strep-Tactin multimer in the presence of low concentrations of d-biotin. Subsequently, the weakly binding monovalent MHCs dissociate spontaneously and rapidly from the T-cell surface.
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| Cat no. | Description | ||
|---|---|---|---|
| 6-7001-005 | MHC I-Strep HLA-A*0201 CMV PP65, NLVPMVATV 50 | ||
| 6-7001-050 | MHC I-Strep HLA-A*0201 CMV PP65, NLVPMVATV 500 | ||
| 6-7002-005 | MHC I-Strep HLA-A*0201 EBV GLCTLVAML 50 tests | ||
| 6-7002-050 | MHC I-Strep HLA-A*0201 EBV GLCTLVAML 500 tests | ||
| 6-7003-005 | MHC I-Strep HLA-A*0201 Influenza M GILGFVFTL 50 | ||
| 6-7003-050 | MHC I-Strep HLA-A*0201 Influenza M GILGFVFTL 500 | ||
| 6-7004-005 | MHC I-Strep HLA-A*0201 HIV gag SLYNTVATL 50 tests | ||
| 6-7004-050 | MHC I-Strep HLA-A*0201 HIV gag SLYNTVATL 500 tests | ||
| 6-7005-005 | MHC I-Strep HLA-A*0201 HIV pol ILKEPVHGV 50 tests | ||
| 6-7005-050 | MHC I-Strep HLA-A*0201 HIV pol ILKEPVHGV 500 tests | ||
| 6-7006-005 | MHC I-Strep HLA-A*0201 HBV core FLPSDFFPSV 50 | ||
| 6-7006-050 | MHC I-Strep HLA-A*0201 HBV core FLPSDFFPSV 500 | ||
| 6-7007-005 | MHC I-Strep HLA-A*0201 MART 1 ELAGIGILTV 50 tests | ||
| 6-7007-050 | MHC I-Strep HLA-A*0201 MART 1 ELAGIGILTV 500 tests | ||
| 6-7008-005 | MHC I-Strep HLA-A*0201 Her-2/neu KIFGSLAFL 50 | ||
| 6-7008-050 | MHC I-Strep HLA-A*0201 Her-2/neu KIFGSLAFL 500 | ||
| 6-7009-005 | MHC I-Strep HLA-A*0201 Her-2/neu RLLQETELV 50 | ||
| 6-7009-050 | MHC I-Strep HLA-A*0201 Her-2/neu RLLQETELV 500 | ||
| 6-7010-005 | MHC I-Strep HLA-A*0201 Gp100 IMDQVPFSV 50 tests | ||
| 6-7010-050 | MHC I-Strep HLA-A*0201 Gp100 IMDQVPFSV 500 tests | ||
| 6-7011-005 | MHC I-Strep HLA-A*0201 Gp100 ITDQVPFSV 50 tests | ||
| 6-7011-050 | MHC I-Strep HLA-A*0201 Gp100 ITDQVPFSV 500 tests | ||
| 6-7012-005 | MHC I-Strep HLA-A*0201 Tyrosinase YMDGTMSQV 50 | ||
| 6-7012-050 | MHC I-Strep HLA-A*0201 Tyrosinase YMDGTMSQV 500 | ||
| 6-7014-005 | MHC I-Strep H-2 Kd LLO 91-99 GYKDGNEYI 50 tests | ||
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