Protein-DNA Binding Assay Kit

Features & Benefits

• No gel electrophoresis
• No radioactive labeling
• Sensitive chemiluminescent ProLabel detection
• 96-well plate format
• Specific and quantitative detection of protein-DNA binding
• Biologically relevant results

Description

The Protein-DNA Binding Assay provides a safe, fast, and sensitive alternative to traditional electromobility shift assays (EMSA) for detection and quantitative characterization of protein-DNA interactions.

The assay is performed in a 96-well plate, eliminating the need for gel electrophoresis. It also abolishes the need for radioactive labeling of nucleic acids because the assay is reformatted to take advantage of Clontech’s sensitive and quantitative ProLabel™ chemiluminescence detection technology. This method consists of fusing a small (~6 kDa) ProLabel tag to your protein of interest. The resulting ProLabel fusion protein is capable of producing a strong chemiluminescent signal via an enzyme complementation assay.

As an additional feature, the ProLabel fusion protein is expressed in mammalian cells, where it can acquire biologically relevant posttranslational modifications that may be necessary for functional DNA binding.