Dendra2 Photoswitchable Fluorescent Protein

Features & Benefits

• Irreversible photoswitching from green to red
• Activated by UV-violet or blue light
• High contrast (4000-fold) between green and red forms
• Switched (red) form is highly photostable
• Matures at a wide range of temperatures
• Monomeric fluorescent protein: suitable for fusion

Description

Dendra2 is a monomeric, green-to-red photoswitchable fluorescent protein derived from octocoral Dendronephthya.

Dendra2 is an ideal tool for real-time tracking protein dynamics (movement, degradation, etc.) and monitoring selective cell fate. Once it is irreversibly switched to its red form, Dendra2 is highly photostable.

Dendra2 matures efficiently both at 20 and 37°C, which makes it applicable to a wide range of experimental systems, from cultured mammalian cells to cold-blooded animals. Mammalian cells transiently transfected with Dendra2 display an evenly distributed green signal (e. g., without aggregation) within 10–12 hr posttransfection. No cell toxicity is observed.