Cell Cycle Fluorescent Reporter Vectors (Fucci)

Features & Benefits

• Easily and precisely track cell-cycle progression in live cells
• Monitor phase transitions in cultured cells or live tissue
• Visualize cell shape during phase transitions
• Label whole cells or just nuclei
• Monitor stably expressed phase reporters over long time periods

Description

Looking for a way to easily and precisely track cell-cycle progression in live cells? Clontech’s cell cycle reporter vectors let you monitor the cell cycle progression of living cells in real-time, without fixation. You can even visualize cell shape.

Fucci Probes
Our cell cycle reporter vectors deliver fluorescent, ubiquitination–based, cell-cycle indicators (Fucci) that allow the identification of cells in various phases of the cell cycle. These Fucci probes contain Cdt1 or Geminin, proteins whose levels fluctuate differentially throughout the cell cycle: Cdt1 levels peak in G1 phase; as cells transition into S phase, Cdt1 levels fall and Geminin levels rise, remaining high until the cells are back in G1.

Cells control Cdt1 and Geminin levels post-translationally, using ubiquitination to target the unwanted proteins for proteasomal degradation. To allow Geminin and Cdt1 to be easily visualized, each is expressed with a red or cyan fluorescent tag. These probes allow precise, visual evaluation of the cell cycle phase.