Clontech PCR-Select cDNA Subtraction Kits

Features & Benefits

• Ideal for isolating novel, differentially expressed genes
• Provides greater than 1,000-fold enrichment of rare transcripts
• Requires only 2 µg of poly A+ RNA
• CDNA subtraction process takes only 3–4 days

Description

The Clontech PCR-Select™ cDNA Subtraction Kit offers an efficient method for selectively amplifying differentially expressed genes—those genes expressed in one mRNA population but reduced or absent in another. This method is particularly well-suited for the identification of target cDNAs that correspond to rare transcripts, typically the most difficult to obtain. In contrast to other methods that require physically separating single-stranded and double-stranded cDNAs, the Clontech PCR-Select™ method allows the exponential amplification of only the desired sequences.

Subtraction can be performed with just 2 µg of poly A+ RNA. This feature is especially useful when working with RNA samples that are difficult to obtain. If you have only nanograms of total RNA, generate high-quality cDNA for use in Clontech PCR-Select cDNA subtraction with the Super SMART™ PCR cDNA Synthesis Kit.

Advantage® 2 Polymerase Mix is recommended for this kit but not included.