Colony Fast-Screen Kits

Description

The Colony Fast-Screen™ Kit (PCR Screen) provides a rapid method for preparing clones for screening by PCR. Using the Colony Fast-Screen Kit (PCR Screen) there is no need to grow cultures or purify DNA prior to PCR. The kit can be used with all standard E. coli hosts and all cloning vectors. Thermostable polymerase and PCR primers are not provided.

The Colony Fast-Screen™ Kit (Size Screen) provides a rapid and sensitive method for estimating the size of cloned DNAs from colonies on primary culture plates without the need to grow cultures, or perform minipreps or restriction endonuclease digestions. The size of most clones can be determined in 1 hour or less. BAC clone sizes can be estimated in as little as 4 hours. The kit provides optimal performance with high-copy-number clones.

The Colony Fast-Screen™ Kit (Restriction Screen) provides an easy and rapid method to screen the size and orientation of cloned DNA by restriction digests, without overnight cultures or plasmid minipreps. The Colony Fast-Screen Kit Restricti-Lyse™ solution lyses cells in an environment which is not inhibitory to restriction enzyme activity. Thus, the kit enables the use of any restriction enzyme for restriction analysis. E. coli recombinant colonies can be screened in 25 minutes using the standard protocol , or in as little as 10 minutes with the accelerated protocol.