EZ-Tn5 In-Frame Linker Insertion Kit

Features & Benefits

• Generation of random 57-bp (19-amino-acid) insertions that are readable in all three reading frames in any cloned DNA that encodes an expressed protein for protein domain mapping, protein engineering, or epitope mapping.
• Generate a population of >10^6 insertion clones, each with a single randomly inserted transposon, to provide complete coverage of the clone
• More versatile than traditional Linker Scanning Mutagenesis: EZ-Tn5 Transposon insertion is random and not limited to pre-existing restriction endonuclease sites in the cloned DNA