In-Fusion HD EcoDry PCR Cloning Kit

Features & Benefits

• Versatile – sequence and restriction-site independent cloning; clone any insert, into any location, within any vector you choose
• Reproducible - dry format reduces variation between experiments
• Efficient – achieve >95% efficiency even when cloning large fragments or multiple fragments Fast – clone one or multiple DNA fragments simultaneously into any vector in a single reaction
• No subcloning necessary
• Simple – no restriction digestion, phosphorylation, or blunt-end polishing; no subcloning
• Directional – the insert is always cloned in the desired orientation
• Precise – final constructs are seamless with no extra or unwanted base pairs

Description

The In-Fusion Cloning System offers directional cloning of any PCR fragment or multiple fragments into any linearized vector in a single 30-minute reaction. No additional treatment of the PCR fragment is required.

With In-Fusion you can clone multiple PCR fragments into any vector without subcloning, create modular expression vectors with interchangeable parts, construct seamless fusion proteins, delete and replace DNA segments, insert point mutations, or swap tags on a gene.

The In-Fusion PCR Cloning system delivers greater than 95% cloning efficiency over a broad range of fragment sizes, even up to 15 kb.

The In-Fusion EcoDry Kits provide In-Fusion enzyme reaction mix in lyophilized (dry-down) format. With our lyophilized kits, the In-Fusion Enzyme comes in a ready-to-use microtube which reduces variations between experiments caused by pipetting errors and enables consistent results. Simply add your PCR fragment and a linearized vector, and incubate.