Thermo-Start ReddyMix Hot-start Master Mix

Features & Benefits

• Gel-ready samples, no need to add gel loading buffer
• Easy sample visualization during reaction set-up.
• Maximum specificity. Efficient amplification of even low copy number targets
• Optional High Performance buffer for amplification of GC-rich sequences and other challenging targets.

Description

Thermo-Start Taq DNA Polymerase is a chemically modified enzyme that remains completely inactive during reaction setup, to prevent non-specific amplification and primer dimer formation. This high performance hot start enzyme is now available in ReddyMix format. ReddyMix PCR buffers have an inert red dye and gel loading precipitant incorporated into the mix. The inert red dye aides in error free pipetting during setup and the precipitant allows for the competed PCR sample to be directly loaded onto an agarose gel. After thermal cycling, a sample of the reaction can be removed and loaded directly onto the gel without the additional step of adding a loading buffer. Combining the components for PCR and a gel-loading buffer into a single master mix eliminates many pre- and post- PCR set-up steps.