QPCR Reference RNA & cDNA

Features & Benefits

• Standardize expression data from different experiments
• High gene representation
• Consistent results
• Made from human tissues, not cultured cell lines

Description

Measurements of mRNA expression levels are usually standardized by comparing the data to an internal reference gene. Housekeeping genes such as beta-actin or GAPDH are most often used because their expression levels are expected to remain constant under different treatment conditions. Unfortunately, this assumption is not always valid, and results based on housekeeping genes alone can be biased.

A better method is to normalize your data using our qPCR Human Reference cDNA. Because it is prepared from a total RNA pool collected from several different tissues, it provides broad gene coverage, as shown by microarray analysis of the RNA starting material. Lot-to-lot variation is minimal because the RNA source is prepared on an industrial scale. Both high and low abundance genes are well represented allowing preparation of a wide range of serially diluted standards for each qPCR assay.