RNAiMonitor

Features & Benefits

• Confirm gene silencing the easy way
• Fast and highly sensitive
• The easier alternative to Western Blots
• No cell lysis

Description

The RNAiMonitor is designed to monitor knockdown of RNAi target sequences efficiently and easily, using a no-cell-lysis protocol.

The pRNAiMonitor-MetLuc2 vector expresses Metridia luciferase (MetLuc), a secreted luciferase reporter that can easily be detected in the medium surrounding the cells. When a target sequence (encoding the target of your shRNA or siRNA) is cloned downstream of the Metridia luciferase gene, a bicistronic mRNA transcript encoding both the Metridia luciferase and the target sequence is produced. Target gene knockdown results in the degradation (or translational inhibition) of the mRNA transcript, which causes a proportional decrease in the expression of the reporter protein. The reporter molecule is easily detected with the Ready-To-Glow Secreted Luciferase Reporter Assay kits.