Adeno-X Adenovirus Expression System 1

Features & Benefits

• Generate recombinant adenovirus for expressing a protein in only a few weeks
• Simple ligation-based system for constructing recombinant adenovirus
• Efficiently express protein in dividing and non-dividing cells

Description

Our protocol has been optimized so that recombinant adenovirus can be generated faster than with other methods. Instead of homologous recombination, this protocol uses an efficient, ligation-based method with pre-linearized, ready-to-ligate Adeno-X System 1 Viral DNA. The system also provides a convenient shuttle vector, pShuttle2, and a unique double digestion buffer that saves time during cloning.

By using a ligation-based approach, the Adeno-X System 1 eliminates nonrecombinant adenovirus. With our optimized protocol, this in vitro ligation approach produces recombinant adenovirus much more rapidly and reliably than conventional homologous recombination approaches without having to switch bacterial strains.