pCold Cold-induced Expression System

Features & Benefits

• High Yield: up to 60% of expressed protein is target protein
• Low-temperature expression increases protein solubility
• Up to 90% of newly expressed protein is target protein: ideal for isotope labeling
• Solubility can be further improved with fusion tags TF or ProS2

Description

Cold-shock expression vectors, pCold™ DNA, are designed to perform efficient protein expression utilizing promoter derived from cspA gene, which is one of the cold-shock genes. At the downstream of the cspA promoter, lac operator is inserted so that the expression is strictly controlled. In addition, 5′ untranslated region (5′ UTR), translation enhancing element (TEE), His-Tag sequence, Factor Xa cleavage site, and multicloning site(MCS) are located at the downstream of the cspA promoter. As this product utilizes the promoter derived from E. coli, most E. coli strains can be utilized as an expression host.
There are four basic pCold™ vectors, whose arrangements vary in the existence of TEE, His-Tag sequence and Factor Xa clevage site.

To increase solubility of your protein of interest even further pCold TF and pCold ProS2 are the best choice.
pCold TF creates a fusion between the target protein and Trigger Factor, a chaperone protein that promotes correct folding - and thereby solubility - of the fused protein.
pCold ProS2 enhances protein solubility by fusion to the highly stable and soluble Protein S from Myxococcus xanthus.