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Protein analysis
Expression Systems
Bacterial Expression
HAT Protein Expression & Purification System
The HAT (histidine affinity tag) epitope is a naturally-occurring sequence of non-adjacent histidine residues that has a lower overall charge than tags with consecutive His residues.
HAT-protein fusions exhibit solubility that more closely resembles that of wild-type proteins, while still possessing strong affinity for immobilized metal ions. Using the HAT System to purify proteins offers two major advantages:
The protein is soluble, therefore it does not form aggregates or reside within inclusion bodies.
The protein can be eluted under mild conditions, such as neutral pH or low imidazole concentration.
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| Cat no. | Description |
|---|---|
| 631205 | HAT Protein Expression&Purification System |
