BacPAK Baculovirus Rapid Titration Kit

Features & Benefits

• Results in 24 hours
• Next-generation, ELISA-based titration method
• No plaques or spots to count
• Suitable for any AcMNPV-type baculovirus

Description

In order to express your protein of interest efficiently in a baculovirus expression system it is crucial to begin with accurate viral stock titers.

The BacPAK Baculovirus Rapid Titration Kit provides a fast and simple method for determining titers of baculovirus stocks. The kit uses a standard immunological assay to accurately determine baculovirus titers within 48 hours, while plaque and end-point dilution assays typically require 5–10 days.

The BacPAK Rapid Titer assay allows titer determination long before plaques are formed. Titers obtained are comparable to those obtained with other methods.

This kit is suitable for use with any virus stock with a titer of more than 10^4 pfu/ml and is compatible with all commonly available (AcMNPV-based) baculovirus expression systems.

The Rapid Titer immunoassay uses a primary monoclonal antibody against an AcMNPV envelope glycoprotein (gp64) to identify virus-infected cells. A secondary HRP-conjugated antibody enables you to visualize infected cells by light microscopy and determine viral titer.