Tet-On 3G Inducible Expression System

Features & Benefits

• Lowest Background
• Highest Sensitivity
• Highest Fold Induction

Description

The Tet-On 3G Tetracycline Inducible Gene Expression Systems are the third generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. They provide precisely regulated control of transgene expression that is reversible, quantitative, and reproducible.

Lowest Background
Mutations within the PTRE3G promoter have reduced background expression from this tetracycline inducible promoter by 5–20-fold compared to our previous tightest promoter PTight .

Highest Sensitivity
Mutations to create the Tet-On 3G transactivator have significantly increased its sensitivity to the inducer doxycycline (Dox)

Highest Fold Induction
Fold induction refers to the difference between the induced and uninduced states. Since Tet-On 3G retains the high maximal expression demonstrated by all Tet-On and Tet-Off systems but has significantly reduced basal expression compared to its predecessors, the fold induction levels are far higher.