Lenti-X Accelerator

Features & Benefits

• Accelerate lentiviral transduction to 25 min
• No Polybrene - low toxicity
• Faster transduction of lentivirus and MMLV retrovirus
• Ideal for sensitive cell types such as stem cells
• Starter Kit includes a magnetic separator

Description

Lenti-X™ Accelerator is a magnetic bead-based technology accelerating transduction of lentivirus and retrovirus. Lenti-X Accelerator is also ideal for sensitive cells, since it limits the amount of time that cells are exposed to viral supernatant to just 5 minutes, compared to an overnight incubation if the cells are treated with Polybrene.

Lenti-X Accelerator contains charged magnetic beads that selectively bind to virus particles from your lentiviral packaging supernatant and are pulled into contact with your cells by a magnet. The magnetic particles result in higher transduction efficiency by creating an increased localized concentration of virus at the surface of your cells.

The Lenti-X Accelerator Starter Kit includes a powerful magnetic separator suitable for all 6-, 12-, 24- or 96-well cell culture dishes.

Lenti-X Accelerator beads are large enough to speed up transduction by gravity alone, without using a magnet. Transduction efficiency without the Magnetic Separator is still very rapid (15 min) compared to Polybrene (overnight). The Magnetic Separator further reduces the transduction time to 5 min and provides 10% higher transduction efficiencies. The Magnetic Separator also allows easy removal of beads from your transduced plate.