Lenti-X qRT-PCR Lentivirus Titer Kit

Features & Benefits

• Determine lentivirus titer in only 4 hours
• Harvest, titer, and infect in a single day
• Kits work with HIV-1-based lentiviral vectors
• Accurate lentivirus titers produce the most consistent infection results

Description

Why you need to know your lentivirus titer:
- Confirm the success of your packaging reaction to avoid wasting time with your experiments
- Perform consistent experiments
- Ensure sufficient MOIs so that 100% of your cells are transduced
- Control copy number and hence expression levels

The Lenti-X qRT-PCR lentivirus titer kit provides an extremely fast and simple method to detrmine the titer of your lentivirus stocks. The titer kits use a quick RNA purification step before determining viral genome content using qRT-PCR and SYBR® technologies. Whereas standard methods to measure lentivirus titer require up to 10 days to complete, these titer kits require only 4 hours and work with HIV-1-based lentivirus vectors.

Using qRT-PCR dramatically shortens the time interval between lentiviral harvest and target cell infection, allowing you to perform both on the same day. You can avoid delays that lead to reduced lentivirus infectivity and can infect target cells at a known multiplicity of infection (MOI) for more consistent results.