Retroviral Expression Vectors

Features & Benefits

• Retroviral vectors designed for high retroviral titers and high expression of your transgene
• Compatible with all of Clontech retroviral packaging cell lines

Description

We offer a choice of Clontech retroviral vectors, retroviral vector sets, or a complete system with retroviral packaging cell line. All retroviral vectors are derived from MMLV. Retroviral vectors transduce a wide range of hard-to-transfect dividing cells. For non-dividing cells we recommend lentiviral or adenoviral vectors.

Q-Series of vectors contain a CMV/MSV hybrid promoter in the 5' LTR to drive high titers during the packaging step. They also contain a self-inactivating 3' LTR to reduce promoter interference and drive high expression of your transgene from the internal CMV promoter. An IRES sequence allows co-expression of an antibiotic selection marker.

The Retro-X Q Vector Set gives you a choice of Q-Series vector backbones with alternative resistance markers for selection with G418 (pQCXIN), hygromycin (pQCXIH), or puromycin (pQCXIP). pQCXIX does not contain a selection marker, but does contain two multiple cloning sites, one on each side of an IRES, to allow simultaneous expression of two transgenes.

pLNCX2 and pLXSN are basic retroviral vectors that utilize wild-type LTRs from MMLV. These allow you to express your gene of interest from either a CMV promoter (pLNCX2) or the 5'LTR promoter (pLXSN). These vectors can be selected with G418.

The Retro-X System includes three L-Series retroviral expression vectors, pLNCX2, pLXSN, and pLAPSN. The latter is a control reporter vector made by adding the bacterial alkaline phosphatase gene to pLXSN. Clontech's RetroPack PT67 packaging cell line is also included in this system.

The LRCX Retroviral Vector Set gives you a choice of L-Series vector backbones with alternative resistance markers for selection with G418 (pLNCX2), hygromycin (pLHCX), or puromycin (pLPCX).

pRetro-Lib contains Sfi1A and Sfi1B restriction sites flanked by a stuffer fragment to enable simple cloning of a full-length cDNA library using Clontech's SMART cDNA Library Construction Kit.