CellVue Dye Kits

Description

CellVue® fluorescent imaging kits use proprietary labeling technology to stably incorporate fluorescent dyes containing long aliphatic hydrocarbon tails into lipid membranes. They are useful for researchers working in all aspects of science and technology where fluorescently labeled cells and/or tissues are required. The molecular structure of CellVue® dyes consist of long aliphatic hydrocarbon tails linked to a polar fluorescent chromophore. These extremely lipophilic fluorescent dyes rapidly and stably integrate into the phospholipid membrane of cells or other membrane-containing bioparticles by non-covalent interactions. The dyes are stably maintained within the lipid bilayer through strong hydrophobic interactions and do not transfer into the unstained membranes of adjacent cells. This characteristic permits a labeled cell to be tracked for extended periods of time. The rapid incorporation of the dye also allows for immediate analysis of cell functions without any waiting period. The pattern of fluorescence staining within the membrane may vary depending on the cell type. All of the dyes are designed to be biocompatible at optimized concentrations.

The CellVue® dyes are provided in an easy to use kit format comprised of a solution of the dye in ethanol and a cell labeling diluent. The labeling diluent provided with the kit is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents, and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The simple membrane labeling protocol provided with the kit is applicable to almost any type of cell or organism.

The excitation and emission properties of CellVue® Burgundy and CellVue® NIR815 dyes are compatible with a range of commercially available near-infrared fluorescent plate readers, flow cytometers, in vitro and in vivo fluorescent imaging systems, and confocal microscopes.

Due to the aforementioned properties, the CellVue® dyes provide the researcher with valuable tools for a multitude of in vivo and in vitro cell studies using fluorescent membrane labels.