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Protein analysis
Protein Interaction
Protein Complex Purification
One-Strep Protein Interaction Set
The new One-STrEP system is an extremely fast and efficient method to identify protein complexes. While other systems require tedious optimization because of high background or two successive purification steps, the One-STrEP system requires just a single step to isolate intact protein complexes with low background. Purification is rapidly performed under physiological conditions enabling even the isolation of weakly associated binding partners.
Cells expressing bait protein fused to the One-STrEP-tag are lysed and the bait together with bound proteins (preys) is isolated using a Strep-Tactin column. Proteins are then resolved on a SDS-gel and specifically bound proteins can be idimentified by mass spectrometry or verified by Western blot using specific antibodies.
| Cat no. | Description |
|---|---|
| 2-1109-000 | One-Strep Protein Interaction Kit |
| 2-1110-000 | One-Strep Follow-up Kit (without controls) |
| 2-1121-001 | One-STrEP Set, mammalian |
| 2-1121-002 | One-STrEP Set, E. coli |
| 5-1621-001 | StarGate One-STrEP Cloning Kit, mammalian |
| 5-1621-002 | StarGate One-STrEP Cloning Kit, E. coli |
| 2-1121-011 | One-STrEP Purification Kit, mammalian |
| 2-1121-012 | One-STrEP Purification Kit, E. coli |
