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One-TAP Protein Interaction Set One-TAP Protein Interaction Set

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Features & Benefits

  • Advantages:
  • Only one tag minimizes influence on bait protein
  • Two independent purification steps increase signal-to-noise ratio
  • Discrimination of non-specific and weak binding partners possible by comparison of different methods (One-STrEP and One-TAP)
  • Well-suited for strong protein:protein interactions
  • Convenient and highly flexible bait cloning procedure available (StarGate®)
  • Disadvantages:
  • Purification with two columns makes method more time-consuming and complex
  • Risk of loosing weak binding partners during second purification step

Description

The One-STrEP™ system is recommended for getting started. It needs one tag and one purification step only and is validated for eukaryotes and prokaryotes. Due to its excellent performance, this method yields a favorable signal-to-noise ratio in most cases. Mild elution and fast washing allow the isolation of even weekly interacting preys.

In case the One-STrEP system provides suboptimal data the "One-TAP" system (One-tag Tandem Affinity Purification with One-STrEP-tag on Strep-Tactin® and StrepMAB-Classic) extends the options of the One-STrEP system since it adds a second independent purification step yet with the same tag. Two different purification steps may better discriminat specific from non-specific binding but bear the risk of loosing weakly interacting partners.

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Ordering information

Cat no. Description
2-1122-001 One-TAP Set, mammalian
2-1122-002 One-TAP Set, E. coli
5-1622-001 StarGate One-TAP Cloning Kit, mammalian
5-1622-002 StarGate One-TAP Cloning Kit, E. coli
2-1122-011 One-TAP Purification Kit, mammalian
2-1122-012 One-TAP Purification Kit, E. coli
2-1010-000 Mammalian PPI Control Donor Vector (bait) 200 ng
2-1592-001 PP2Ac affinity-purified Goat Polyclonal Ab 50µl
2-1009-000 E. coli PPI Control Donor Vector (bait); 200 ng
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