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Protein analysis
Protein Interaction
Protein Complex Purification
Two-TAP Protein Interaction Set
The "Two-TAP" system (Two-tag Tandem Affinity Purification with One-STrEP-tag on Strep-Tactin® and FLAG®-tag on M2 mAb) is recommended only as an option in case of unsatisfying data with the One-STrEPTM or "One-TAP" approach and not as first choice starting point (although a successful approach has already been published). Two different tags increase the risk of non-specific binding or interference with the native conformation of the bait necessary for an effective binding of associated proteins.
As the highly charged metal ion binding FLAG®-tag bears the risk of non-specific interactions, – in fact, metal ions are often part of active centers of enzymes and involved in signalling cascades - this system is recommended only if the performance of One-TAP for a given protein complex is insufficient. In the first step, One-STrEP/FLAG® double-tagged proteins are eluted from Strep-Tactin® Superflow® under native conditions with biotin, in the second step elution from anti-FLAG M2 agarose is performed with the FLAG octapeptide.
| Cat no. | Description |
|---|---|
| 2-1123-001 | Two-TAP Set, mammalian |
| 2-1123-002 | Two-TAP Set, E. coli |
| 5-1623-001 | StarGate Two-TAP Cloning Kit, mammalian |
| 5-1623-002 | StarGate Two-TAP Cloning Kit, E. coli |
| 2-1123-011 | Two-TAP Purification Kit, mammalian |
| 2-1123-012 | Two-TAP Purification Kit, E. coli |
