Easy Yeast Plasmid Isolation Kit

Features & Benefits

• Easy spin column prep
• Purify more DNA in less time
• Zymolyase efficiently digests cell wall

Description

The Easy Yeast Plasmid Isolation Kit provides a simple and efficient method for rescuing plasmid DNA from yeast (Saccharomyces cerevisiae). The protocol uses
Zymolyase to efficiently digest the cell walls of the yeast and generate spheroplasts, which are then subjected to SDS/alkaline lysis. A spin column purifies the plasmid DNA, which can then be transformed into E. coli for propagation and scaled-up plasmid preparations, or used as a template for PCR.