Stabilizor T1 Proteome Stabilization System

Features & Benefits

• Eliminate protease and phosphatase activity completely and permanently
• Preserve the true protein molecular weight and phosphorylation state
• No addition of inhibitors required
• Standardize sample handling to improve reproducibility
• Maintain sample stability and composition during transport and storage
• Accurate sample comparison and data interpretation downstream

Description

Protein and peptide degradation begin within seconds of a tissue being sampled. As a consequence, vital information about the original biological state may be destroyed or distorted, leading to inter-sample variation and the risk of incorrect data interpretation. Conventional ways of inhibiting protein degradation, such as inhibitor cocktails, pH changes or cross-linking, have several disadvantages including interference with downstream analysis, reversibility, toxicity or impracticality.

Denator’s heat inactivation technology employs highly efficient, reproducible heating under pressure. This combination ensures rapid, homogenous thermal denaturation of proteins in a tissue sample resulting in complete, permanent inactivation of all enzymes.

The procedure is easy and fast. Tissue samples are placed in a Maintainor Tissue card. To enhance heat transfer, Maintainor Tissue cards are constructed with fluoropolymer foils and air in the sample chamber is evacuated before heat stabilization begins. This also prevents oxidation during stabilization and storage. The Stabilizor T1 system measures sample size automatically to determine the exact treatment time.

Applications include:
• Proteomics
• Peptidomics
• Phosphoproteomics