LICOR Odyssey CLx Western Blot Detection System

Features & Benefits

• Rely on the standard in Western detection for 10+ years
• Produce better data with quantitative Westerns
• Dynamic range up to 6 logs
• Get equal or better sensitivity than film
• Lower your Western blot running costs by omitting film and reagents
• Automatically get an excellent exposure on the first try

Description

Over the last ten years, the LI-COR Odyssey Imager has revolutionized quantitative Western blot research. Carrying on that tradition, the new Odyssey CLx provides all the excellence of the classic Odyssey, but adds easier-to-use software and an AutoScan function for better images without saturation and a broader dynamic range (6 logs).

Direct detection on the Odyssey Infrared Imaging System has become the established standard for Western blot analysis. Infrared detection gives you the quantitative Western analysis and wide linear dynamic range that chemiluminescence and visible fluorescence cannot offer.

The Odyssey Western Detection System gives you clear, sharp, reproducible bands and no blown out blobs. Strong and weak bands on the same blot are accurately detected without the uncertainty and inconvenience of multiple exposures, and without spending time in the darkroom. Bands hidden by overexposure with chemiluminescence are clearly visible in Odyssey images.

In the visible wavelength range used by most fluorescent imaging systems, membranes and plastics produce high background due to light scattering and autofluorescence. This high background limits the sensitivity of visible fluorescent systems and makes it nearly impossible to detect lowabundance proteins at endogenous levels. At the infrared wavelengths detected by Odyssey, both autofluorescence and light scatter are dramatically reduced. The result is the cleanest background, highest signal-to-noise ratios, and best detection sensitivity available with a fluorescent system.

The two infrared fluorescent detection channels of the Odyssey System enable simultaneous two-color target analysis – an advantage that’s not available with chemiluminescent or radioactive methods. Two-color Western blot analysis makes normalization easy and eliminates error introduced by stripping and reprobing or by comparison of separate blots. Superior image clarity and detail make it easier to detect subtle mobility shifts caused by protein modifications such as phosphorylation.

The accuracy and linearity of the Odyssey System detection allows you to be confident about differences you see in protein levels, and your blots can be archived and imaged again months later, if needed.