Odyssey Blocking Buffers

Features & Benefits

• Odyssey Blocking Buffer: the gold standard for quantitative Western blot detection
• Contains no mammalian proteins, for the lowest cross-reactivity with mammalian antibodies
• Ready-to-use formulation in PBS
• Suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays
• Milk-based blocking buffers also available for other applications

Description

LI-COR offers several blocking buffers for use with the Odyssey® and Aerius® Systems. All are optimized for use with IRDye® products and other near-infrared fluorophores, and provide excellent performance for quantitative Western blots and other immunoassays.

Blocker choice is important for immunoassay success. Blocking buffers enhance sensitivity by reducing background interference, increasing signal-to-noise ratio, and promoting specific binding of the primary antibody while minimizing non-specific interactions.


- Insufficient blocking yields high background and reduced signal-to-noise ratios.
- Excessive blocking may cause loss of blotted proteins1, or mask antibody:antigen interactions.
- Detection reagents may cross-react with certain blocking buffers.
- No single blocking buffer selection is suitable for ALL antigen-antibody pairs. Blocker choice may affect antibody specificity and nonspecific binding.
- Empirical testing is critical! The Blocking Buffer Sample Pack provides smaller quantities of all three blockers, to help you identify the best choice for your antigen.