Odyssey In-Cell Western Kits

Features & Benefits

• Sensitive, quantitative analysis of cellular signaling pathways
• “Snapshot” of signal transduction or protein expression of cell population in each well
• Higher throughput: no cell lysis, gel electrophoresis or membrane transfer
• Higher precision
• Easily screen effects of cell treatments or drug candidates

Description

The In-Cell Western Assay is an immunofluorescent assay that uses NIR fluorescence to detect and quantify proteins in fixed cells. Detecting proteins in their cellular context increases quantification precision.

Proteins in fixed, cultured cells are detected directly in microplates, which yields higher throughput compared to Western blotting and eliminates typical Western blotting steps, such as cell lysate preparation, electrophoresis, and membrane transfer.