Film vs Digital: A Western detection battle in 4 rounds
Film supremacy challenged by a new kid on the blot
The supremacy of film for chemiluminescent Western detection is challenged by the C-DiGit Western Blot Scanner, Discover how the C-DiGit gives film the knockout blow in four rounds.
Round #1: Ease-of-use
To get a good image on traditional film you have to do a number of tedious steps that include messing around with toxic chemicals in the darkroom. When you finally have the result, you still need to scan it to get it in digital form.
The C-DiGit is a simple, clean benchtop Western blot scanner. Place your blot on the scanner, press the button, and get your image in a single scan, ready for analysis and publication. You skip the darkroom, and get your results immediately.
The C-DiGit clearly takes the lead after Round #1.
Round # 2: Get the right exposure
With film you usually have to do multiple exposures to get the right one. Not with the C-DiGit. A single scan records a full range of exposures, so you always have the right one. That's 2-0 in favor of the C-DiGit.
Round #3: dealing with strong and faint signals
When film is used to detect chemiluminescent signals, band resolution and clarity are often poor. Strong signals blur and diffuse on film, and these blobs invade adjacent bands. This “blowout” effect, is especially problematic when strong and faint signals must be detected on the same blot. The long exposures you need to detect faint signals increase blowout of strong signals.
Clarity and resolution are much better in C-DiGit images. Stronger bands do not spread out obscuring bands next to them. In addition, bands that are close to each other are visible separately.
So finally you can accurately capture both strong and faint bands in a single exposure, which is impossible with film. Film can hardly make it to the last round...
Round #4: Running cost
The running cost of film-based Western blot imaging is high, and continues to rise. Much less film is produced and the cost of raw materials has risen tremendously. But it's not just film. You also pay for developing chemicals, waste disposal, gloves and maintenance of the always-breaking-down developer unit. And the darkroom itself takes up scarce lab space.
Keep all that money in your pocket with the C-DiGit. Just add your chemi substrate and scan. That's all there is to it! And it's the final knockout for film.
Impressed with the winner? Learn more!
Simply provide your name and email below and get a C-DiGit info package in your Inbox.
You will get several application notes and a brochure.