GeniePlex Quantitative Multiplex ELISA assays on your Flow Cytometer

What are GeniePlex Multiplex Kits

Genieplex kits are custom or premixed multiplexed bead-based immunoassay panels that allow you to assay up to 24 analytes using as little as 15μL sample on standard flow cytometers.

GeniePlex benefits

  • Analyze: Multiplex 2-24 analytes in a single 15ul sample in 2 hours

  • Wide Range of sample types, including cell culture supernatants, saliva, plasma, cell/tissue lysates, serum, BALF, pleural & peritoneal fluids

  • Sensitive: Measure as low as <10 pg/ml of each analyte

  • Dynamic: Lower limit < 20 pg/mL | Upper limit > 5,000 pg/mL

  • Flexible: Assays can be run on most validated flow cytometers (PE & either PE-Cy5 or APC detectors)

  • Validated: All assays fully tested for cross-reactivity

  • Premixed or custom-made: choose from 400 analytes

Download the brochure

Genieplex principle and procedure

GeniePlex is a bead-based multiplex immunoassay technology that uses multiple bead populations differentiated by size and fluorescence intensity. This allows quantitation of up to 24 analytes in a single reaction. Samples as small as 15µl can be easily read on most validated flow cytometers equipped with either a single 488nm laser or dual 488 and 633/640 lasers.

The assay procedure consists of a 60-minute antigen and capture antibody conjugated bead incubation step, a 30-minute biotinylated detection incubation step and a 20-minute streptavidin-PE incubation step.

GeniePlex workflow (Click to enlarge) GeniePlex workflow (Click to enlarge)

Get a Premixed Kit or compose your own from 400 analytes

You can choose from 400 assays for human, mouse, rat, porcine, canine & primates. Find your premixed panels to measure Cytokines, Chemokines, Inflammation, Th1/Th2 & Th1/Th2/Th17.

Download brochure with full list of analytes

See all premixed multiplex kits.

Not finding what you need? We also offer custom multiplex assay panel design and development. You can contact us for further details.