- Quantitation of ATPase/GTPase activity < 30 minutes
- High sensitivity: detection of 0.007 U/L
- Fast and convenient: “mix-and-measure” assay
- Amenable to HTS
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate ion.
This ATPase/GTPase Assay Kit offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 – 660 nm).