Caspase-1 Assay Kits
Fluorometric Caspase-1 Assay
The Caspase-1/ICE Fluorometric Assay Kit is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). When Caspase-1 cleaves YVAD-AFC the released AFC emits a yellow-green fluorescence which can be quantified at 505 nm with a fluorescence microplate reader.
Colorimetric Caspase-1 Assay
The Colorimetric version of the Caspase-1 assay is based on generation of the chromophore p-nitroanilide (pNA) by Caspase-1 cleavage of the substrate YVAD-pNA. The pNA light emission can be quantified at 400 or 405 nm with a spectrophotometer or microplate reader.