GenieGlow Luminescent Cytotoxicity Assay Kit

  • Non-desctructive: assay culture medium
  • Highly sensitive
  • Automatable
  • Results in 30 minutes

Luminsecent assay of AK activity

Adenylate Kinase (AK) is an enzyme that occurs in all eukaryotic and prokaryotic cells. In the case of cytotoxicity the plasma membrane becomes leaky, releasing Adenylate Cyclase into the surrounding medium. Therefore AK enzyme activity can be used as a quantitative measure of cytotoxicity.

The GenieGlow Luminescent Cytotoxicity Assay Kit quantifies AK activity in cell culture medium in a simple one-step procedure involving two chemical reactions. First, ADP is converted to ATP by the adenylate kinase that was released from the damaged cells. In the second reaction luciferase catalyzes the generation of light from ATP and luciferin. The light intensity can be measured using a luminometer or beta counter.

Assay protocol

  1. Treat cultured cells according to your experimental question. Include a no-treatment control culture
  2. Transfer 100 μl of the culture medium into a 96 well plate
  3. Add 100 μl of the reconstituted AK assay reagent to each well
  4. Incubate for 5 minutes
  5. Read in a Microplate Luminometer within 30 minutes after addition of the AK assay reagent

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Assay Genie website to find your local distributor.
Cat no. Description
RG BN00579 GenieGlow Cytotoxicity Assay Kit (luminescence) - 500 Assay