- For quantitative determination of molecules involved in glutathione metabolism
- Highly sensitive and accurate
- Simple and convenient assays
- Fast, colorimetric assays
Glutathione S-transferase (GST) activity assay kit
Assay Genie’s colorimetric Glutathione S-transferase, GST, activity assay kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene), and can be used to determine the GST activity in biological samples The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.
Glutathione assay kit
This kit is perfect for the quantitative determination of reduced glutathione (GSH) in biological samples. This colorimetric Glutathione (GSH) Assay Kit is an improved 5,5’-dithiobis(2-nitrobenzoic acid (DTNB) method that combines deproteination and detection (Reagent A) into one reagent. DTNB reacts with reduced glutathione to form a yellow product. The optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample.
To accurately measure total, reduced and oxidized glutathione, we recommend to use the colorimetric GSH/GSSG Assay Kit. You can use Meta-Phosphoric Acid (cat. # RG BA0162) for sample deproteination with the GSH/GSSG Assay Kit.
Glutathione peroxidase activity assay kit
This kit is suitable for quantitative determination of glutathione peroxidase activity and the evaluation of drug effects on GPX activity. Assay Genie's glutathione peroxidase assay directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340 nm is directly proportional to the enzyme activity in the sample.
Glutathione reductase assay kit
Glutathione reductase reduces oxidized glutathione (GSSG) to the reduced sulfhydryl form GSH which is an important cellular antioxidant. Assay Genie's non-radioactive, colorimetric Glutathione Reductase Assay Kit is designed to accurately measure glutathione reductase (GR) activity in biological samples with a method that utilizes Ellman’s method in which DTNB reacts with the GSH generated from the reduction of GSSG by glutathione reductase in a sample to form a yellow product (TNB2-). The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione reductase activity in the sample.