Superoxide Dismutase activity assay kit
- For quantitative determination of superoxide dismutase (SOD) enzyme activity
- Sensitive and accurate.
- Linear detection range of 0.05 – 3 U/mL SOD
- Convenient “mix-incubate-measure” type assay
- Can be readily automated on HTS liquid handling systems
Superoxide dismutases are enzymes that catalyze the dismutation of superoxide into O2 and H2O2. They are an important antioxidant defense in all cells exposed to O2.
This superoxide dismutase activity assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O2–) is provided by xanthine oxidase (XO) catalyzed reaction. O2– reacts with a WST-1 dye to form a colored product. SOD scavenges the O2– thus less O2– is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.