alpha-Mannosidase activity assay kit
- For quantitative determination of alpha-Mannosidase enzyme activity using a p-nitrophenol based substrate
- Fast and sensitive
- Linear detection range (10 μL sample): 1 to 250 U/L
- Results within 10 minutes
- For biological samples e.g. plasma, serum, tissue and culture media
alpha-mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. alpha-mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome.
This non-radioactive, colorimetric alpha-Mannosidase activity assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.