D- Mannitol assay kit
- For quantitative determination of D-mannitol
- Fast and sensitive: linear detection range 0.007 to 3 mM
- Convenient: “Add-mix-read” type assay
- Room temperature assay
This D-mannitol colorimetric assay kit is developed for quantitative determination of D-mannitol in a wide variety of sample types, including biological, food, beverage and agricultural products.
This assay is based on mannitol dehydrogenase catalyzed oxidation of D-mannitol, which generates D-fructose and NADH that reduces a formazan (MTT) dye. The intensity of product color, measured at 565 nm, is directly proportional to D-mannitol concentration in the sample.
- Read-out at 565nm
- Results within 30 minutes
- Use of 20 μL sample
- Can be readily automated as a high-throughput 96-well plate assay