Glucose metabolism kits
- For quantitative determination of glucose metabolism molecules
- Fast, sensitive and accurate
- For a wide variety of sample types
- Simple and convenient: results within 10 min.
- Colorimetric or fluorometric assays
The major pathways of glucose metabolism are glycolysis and the pentose-phosphate pathway. We provide simple and convenient assays for the quantitative determination of molecules from both pathways.
Glycolysis is the metabolic pathway that converts glucose into pyruvate. The first step in glycolysis is phosphorylation of glucose to form glucose 6-phosphate (G6P). G6P has three primary fates within the cell. It lies at the start of both glycolysis and the pentose phosphate pathway. In addition, glucose 6-phosphate may also be converted to glycogen or starch for storage.
Alpha-glucosidase breaks down starch and disaccharides like maltose to glucose.
In humans, excess glucose is converted to glycogen via glycogenesis, which primarily occurs in the liver, skeletal muscles, and kidney. Glycogen stored in the liver can function as a backup source for energy.
Pentose phosphate pathway
The pentose phosphate pathway is a metabolic pathway parallel to glycolysis and is an alternative method of oxidizing glucose. It occurs in the liver, adipose tissue, adrenal cortex, testis, milk glands, phagocyte cells, and red blood cells. The pentose phosphate pathway is regulated through changes in the activity of glucose-6-phosphate dehydrogenase (G6PD).
Fast and accurate quantitative glucose metabolism assay kits
With our kits, you can quantitatively determine glucose concentrations in your samples or glucose uptake by cells in your samples. We offer various kits quantitatively determine all sorts of molecules in the glycolysis and pentiose phosphate pathways,
All kits are based on a specific color reaction that can be measured at indicated wavelengths. Outcomes are directly proportional to the molecule concentration or enzyme activity in the sample.
- Sensitive and accurate. Use as little as 5-20 μL samples.
- Convenient: "Add-Incubate-Read" assays.
- Some kits are compatible with high-throughput liquid handling instruments for HTS.