Krebs (TCA) cycle

  • For quantitative determination of components of the Krebs (TCA/CAC) cycle
  • Sensitive: wide detection ranges
  • Convenient and fast protocols
  • Most assay kits are suitable for high throughput

Malate is a dicarboxylic acid that plays an important role in the Krebs Cycle. It is an intermediate that forms from fumarate in the Krebs Cycle.

Malate dehydrogenase (MDH) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD.

Fumarase is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate.

Isocitrate is a substrate in the citric acid (TCA) cycle and is formed by the isomerization of citrate. It is oxidized by isocitrate dehydrogenase (IDH) producing alpha-ketoglutarate and generating NADPH.

The Krebs (Citric Acid) cycle The Krebs (Citric Acid) cycle

L-malate (L-malic acid) assay kit

Assay Genie’s L-malate assay kit is based on malate dehydrogenase catalyzed oxidation of malate in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm is proportional to the malate concentration in the sample.

Malate is frequently used in food and beverage industries as an additive in products such as wine, beer, candies, and more.

Malate Dehydrogenase assay kit

Assay Genie’s non-radioactive, colorimetric Malate Dehydrogenase assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Fumarate assay kit

Fumarate (Fumaric Acid) is one of the key components in TCA cycle and is used by cells to form ATP.

This fumarate assay kit is based on fumarase catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm is proportional to the fumarate concentration in the sample.

Fumarase enzyme activity assay kit

Assay Genie’s non-radioactive, colorimetric fumarase assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Isocitrate/IDH assay kit

The isocitrate assay measures the NADPH generated from the oxidation of isocitrate, while the IDG assay kit  measures the activity of the NADP+ isoforms via IDH. The converted dyes have an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration or IDH activity.

 

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Assay Genie website to find your local distributor.
Cat no. Description
RG BA0038 Isocitrate Dehydrogenase Assay Kit - 100 assays
RG BA0089 Isocitrate Assay Kit (Colorimetric) - 100 assays
RG BA0105 Fumarase Activity Assay Kit (Colorimetic) - 100 assays
RG BA0110 Fumarate Assay Kit - 100 assays
RG BA0129 Malate Assay Kit - 100 assays
RG BA0131 Malate Dehydrogenase Assay (Colorimetric) - 100 assays