Lactate metabolism kits
- For quantitative determination of lactate metabolism molecules
- Sensitive and accurate
- Room temperature assays
- Can be readily automated as a high-throughput 96-well plate assay
- Fast: results within 20-30 min.
Lactate is constantly produced from pyruvate by the enzyme lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions.
Lactate is continually formed, even at rest and during moderate exercise. However, during power exercises when the rate of demand for energy is high, glucose is broken down and oxidized to pyruvate, and lactate is then produced from the pyruvate faster than the body can process it, casuing a rise in lactate concentrations.
In nature, lactate exists in two isoforms: L-lactate and D-lactate. Both forms are produced from and metabolized to pyruvate by the action of the enzyme lactate dehydrogenase (LDH). Mammalian cells only contain L-LDH so that in humans the lactate produced is almost exclusively L-lactate.
Fast and accurate quantitative lactate assay kits
With our kits, you can quantitatively determine the concentration of L-Lactate, D-lactate or Lactate Dehydrogenase (LDH) in your samples.
With our glycoloysis assay kit you can execute direct determination of L-Lactate produced by glycolysis in cell samples and thus screen for glycolysis inhibitors.
For all kits, the intensity of the reduced dye, measured at 565 nm, is directly proportional to the lactate concentration or enzyme activity in the sample.
Some kits are compatible with high-throughput liquid handling instruments for HTS.