Sialic acid assay kit
- For quantitative determination of free and total sialic acid (NANA)
- Sensitive and accurate
- Use as little as 60 μL sample
- Colorimetric and fluorimetric assays
- For samples types like serum, plasma, saliva, milk and more
Sialic acid is a general name for nine carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum.
This sialic acid assay kit has a linear detection range in 96-well plate of 5 to 1000 μM sialic acid for colorimetric assays and 0.5 to 100 μM for fluorimetric assays. In this kit, sialic acid is oxidized to formylpyruvic acid which reacts with thiobarbituric acid to form a pink colored product.
The color intensity at 549 nm or fluorescence intensity at Ex/Em = 555/585 nm is directly proportional to sialic acid concentration in the sample.