Direct Glucose Uptake Assay
- Direct method provides accurate data
- No glucose analogues: no off-target effects
- Easy to use
- Highly sensitive
The Direct Glucose Uptake Assay kit is simple, ultra-sensitive and easy to use. It makes use of an inhibitor of hexokinase, the first enzyme metabolizing glucose, to arrest glucose conversion after its uptake.
Glucose uptake is then measured by enzymatic oxidation of glucose, producing intermediates that react with the OxiGenie Probe. This generates a fluorescence signal (Ex/Em=535/587 nm) that is directly proportional to the amount of glucose that has been taken up and accumulated inside the cells.
This assay method offers a true measure of natural glucose uptake by the cells and avoids off-target & non-specific results that can be found with 2DG.