AMPK phosphorylation assay kit
- For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells
- Can measure pAMPK modulation in as little as 500 cells/well
- No cell lysis necessary
- Compatbile with human, mouse, rat samples
The 5-AMP-activated protein kinase (AMPK) is a key sensor of intracellular energy balance and is activated in response to an increase in the AMP/ATP ratio.
Assay Genie’s AMPK phosphorylation assay kit measures phosphorylated AMPK in whole cells and normalizes the signal to the total protein content. This AMPK assay does not require cell lysate preparation and can be used to study AMPK regulation in short-term and long-term assays. In this assay, cells grown in 96-well plates are fixed and permeabilized in the wells. Read-out is done at FL360/450nm, 530/585nm.