ERK phosphorylation assay kit
- Quantitative fluorescent immunoenzymatic assay of ERK1/2 phosphorylation status in cultured cells
- Safe, non-radioactive assay
- Determination of total and pERK in the same sample
The MAPK/ERK cascade presents many interesting drug targets for the development of cancer therapies. This ERK phosphorylation assay measures dually phosphorylated ERK1/2 in whole cells and normalizes the signal to the total protein content. There is no need for cell lysate preparation and can be used to study kinase signaling and the effects of kinase inhibitors on cells.
In this assay, cells are grown in 96-well plates and treated with ligands or drugs. Cells are then fixed and permeabilized in the wells. ERK1/2 phosphorylation (pERK) using a fluorescent ELISA followed by total protein reading at ex/em = 530/585 nm and at ex/em =360/450 nm.