NFkB phosphorylation assay kit
- Quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells
- No cell lysis necessary
- NFkB phosphorylation in human or mouse samples
Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-alpha.
Assay Genie’s NFkB phosphorylation assay kit measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. There is no need for cell lysate preparation and this assay can be used to study NFkB regulation in short-term and long-term assays.
Read-out is done by a fluorescence plate reader capable of reading at ex/em = 530/585 nm and at ex/em =360/450 nm.