Free fatty acid kit
- For quantitative determination of fatty acid
- Use 10 μL samples
- Sensitive detection ranges: colorimetric 7–1000 μM, fluorometric 7–100 μM
- Room temperature “mix-and-read” procedure
Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis and energy metabolism.
Assay Genie’s free fatty acid kit provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.