Phospholipid/phospholipase assay kits

  • For quantitative determination of Phospholipids and phospholipase-D activity
  • Use 10-20 μL samples
  • Wide linear detection ranges
  • Can be readily adapted to high-throughput assays for drug screening
  • Colorimetric and fluorometric phospholipid assays

Phospholipase-D activity assay kit

Phospholipase-D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup.

In Assay Genie’s phospholipase-D activity assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.

Phospholipid assay kit

The phospholipid assay kit provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Assay Genie website to find your local distributor.
Cat no. Description
RG BA0143 Phospholipid Assay Kit - 100 assays
RG BA0144 Phospholipase D Activity Assay Kit - 100 assays