Coenzyme A (CoA) assay kit

  • Quantitative measurement of Coenzyme A activity
  • Sensitive: use as little as 10 μL samples
  • Convenient: room temperature “mix-and-read” type assay

Sensitive assay for Coenzyme A activity

Coenzyme A is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of coenzymes A most crucial roles is the carrying and transferring of acyl groups.

In this Coenzyme A assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the coenzyme A concentration in the sample.

  • Linear detection range: colorimetric assay 5 – 1000 μM, fluorimetric assay 3 – 100 μM CoA.
  • Can be readily automated for high-throughput assay of thousands of samples per day.
CoA Assay standard curve


Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Assay Genie website to find your local distributor.
Cat no. Description
RG BA0093 Coenzyme A (CoA) Assay Kit (Colorimetric/Fluorometric) - 100 assays