NAD/NADH assay kit

  • For sensitive determination of NAD and NADH
  • Convenient: "add-mix-read" type assay
  • Results within 15 minutes
  • Room temperature assay

Colorimteric NAD/NADH assay kit

Our colorimteric NAD/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD/NADH concentration in the sample.

This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.

  • Detection limit of 0.05 μM
  • Linearity up to 10 μM NAD+/NADH
  • Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day
  • To determine NAD+/NADH levels in cell- or tissue extracts

Fluorometric NAD/NADH assay kit

Our fluorometric NAD/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample.

This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.

  • Detection limit of 0.02 μM
  • Linearity up to 1 μM NAD+/NADH
  • 10 minutes "add-mix-read" assay
  • Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Assay Genie website to find your local distributor.
Cat no. Description
RG BA0067 NAD/NADH Assay Kit (Colorimetric) - 100 assays
RG BA0106 NAD/NADH Assay Kit - 100 assays