Acid phosphatase (ACP) activity assay kit
- For acid phosphatase activity determination in biological samples
- Sensitive: linear detection range 0.05 - 60 U/L
- Convenient “mix-incubate-measure” type assay
- Suitable for HTS liquid handling systems
- Fast 30 minutes assay
Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes, bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma.
Assay Genie’s non-radioactive, colorimetric acid phosphatase activity assay is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.